480. FERROVUM MYXOFACIENS MEDIUM (FMM)

Basal salt solution (see below) 20.0 ml

Trace elements solution (see below) 1.0 ml

Ferrous sulfate solution (pH 2.0) 5.0 mM

Deionizated water 1000 ml

Adjust pH 2.1 and autoclave at 121oC

Basal salt solution:

MgSO₄ x 7 H₂O 25.0 g

(NH₄)₂SO₄ 22.5 g

Na₂SO₄ x 10 H₂O 7.5 g

KH₂PO₄ 2.5 g

KCl 2.5 g

Ca(NO₃)₂ x 4 H₂O 0.7 g

DI Water 1000 ml

Trace elements solution:

ZnSO₄ x 7 H₂O 10.0 g

CuSO₄ x 5 H₂O 1.0 g

MnSO₄ x 4 H₂O 1.0 g

CoSO₄ x 7 H₂O 1.0 g

Cr₂(SO4)₃ x 15 H₂O 0.5 g

H₃BO₃ 0.6 g

Na₂MoO₄ x 2 H₂O 0.5 g

NiSO₄ x 6 H₂O 1.0 g

Na₂SeO₄ x 10 H₂O 1.0 g

Na₂WO₄ x 2 H₂O 0.1 g

NaVO₃ 0.1 g

DI Water 1000 ml

Updated: 07.01.2024