484. THERMOPLASMA MEDIUM (TERMOplM)

KH₂PO₄ 3.00 g

MgSO₄ x 7 H₂O 1.00 g

CaCl₂ x 2 H₂O 0.25 g

(NH₄)₂ SO₄ 0.20 g

Yeast extract 1.00 g

Glucose 5.00 g

Sulfur 4.00 g

Meat extract 0.50 g

Distilled water 1000.00 ml

Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. Check pH to be 2 to 3. For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N₂ and CO₂ (80: 20, v/v) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract, glucose, and meat extract only are added from separately sterilized stock solutions.

Updated: 07.01.2024