487. ACETICELLA AUTOTROPHICA MEDIUM (ACAUM)

NH₄Cl 0.33 g

KCl 0.33 g

MgCl₂ x 2H₂O 0.33 g

CaCl₂ x 6H₂O 0.33 g

KH₂PO₄ 0.33 g

Na₂S x 9H₂O 0.6 g

Trace element solution (see below) 1 ml

Vitamin solution (see below) 1 ml

Resazurin 1.0 mg

Distilled water 1000.0 ml

Trace element solution:

(NH₄)₂Fe(SO₄)₂ x 6 H₂O (Mohr's salt) 784.0 mg

HCl (concentrated) 5.0 ml

CoCl₂ x H₂O 238.0 mg

(NH₄)₂Ni(SO₄)₂ x 6 H₂O 395.0 mg

Na₂MoO₄ x 2 H₂O 24.0 mg

Na₂WO₄ x 2 H₂O 33.0 mg

ZnSO₄ x 7 H₂O 144.0 mg

CuCl₂ x 2 H₂O 2.0 mg

Na₂SeO₄ 94.0 mg

H₃BO₃ 6.0 mg

MnCl₂ x 4 H₂O 99.0 mg

Distilled water 995.0 ml

Mohr's salt is dissolved firstly in concentrated HCl and mixed with distilled water and other salts are dissolved in the sequence indicated.

Vitamin solution:

Biotin 20.0 mg

Folic acid 20.0 mg

Pyridoxine 0.1 mg

Riboflavin 50.0 mg

Pantotenoic acid 50.0 mg

p-Aminobenzoic acid 50.0 mg

Thiamine-HCl 50.0 mg

Nicotinic acid 50.0 mg

Vitamin B₁₂ 1.0 mg

Lipoic acid 50.0 mg

Distilled water 1000.0 ml

The medium was dispensed in 5-ml portions into 15-ml Hungate’s tubes under 100% CO₂ and the headspace was filled with H₂ and CO₂ (2:1). Na₂S x 9H₂O was used as a reducing agent, To adjust the pH of the medium to 6.0, 3N HCl and 10% NaOH were added.

Updated: 08.01.2024