500. BLUE-GREEN MEDIUM WITHOUT SODIUM NITRATE (BG-11 (-N))

Solution 1:

K₂HPO₄ 0.8 g

Deionised water 200.0 ml

Solution 2:

MgSO₄ x 7H₂O 1.5 g

Deionised water 200.0 ml

Solution 3:

CaCl₂ x 2H₂O 0.72 g

Deionised water 200.0 ml

Solution 4:

Citric acid 0.12 g

Deionised water 200.0 ml

Solution 5:

Ferric ammonium citrate 0.12 g

Deionised water 200.0 ml

Solution 6:

Na₂ЭДТА x 2H₂O 0.02 g

Deionised water 200.0 ml

Solution 7:

Na₂CO₃ 0.4 g

Deionised water 200.0 ml

Trace metals solution:

H₃BO₃ 0.572 g

MnCl₂ x 4H₂O 0.362 g

ZnSO₄ x 7H₂O 0.044 g

Na₂MoO₄ x 2H₂O 0.078 g

CuSO₄ x 5H₂O 0.0158 g

Co(NO₃)₂ x 6H₂O 0.00988 g

Deionised water 200.0 ml

All solutions should be stored unsterilised at 4 C.

Prepare the medium by adding successively 10 ml of each of the first seven solutions and 1 ml of Trace metals solution to 900 ml of Deionized water. Bring total volume to 1 L with Deionised water. Add 14.0 g of Agar.

Autoclave for 20 minutes at 121°C.

Updated: 17.04.2024