61. MEDIUM FOR NITROGEN-FIXING SPIRILLUM

K₂HPO₄ 0.1 g

KH₂PO₄ 0.4 g

MgSO₄ × 7 H₂O 0.2 g

NaCl 0.1 g

CaCl₂ 0.02 g

FeCl₃ 0.01 g

Na₂MoO₄ × 2 H₂O 0.002 g

Na-malate 5.0 g

Yeast extract 50.0 mg

Distilled water 1000.0 ml

pH 7.2-7.4

Autoclave at 121°C for 15 min.