75. MODIFICATION OF TWEEN-80 MEDIUM FOR MILK-ACID BACTERIA

Yeast extract 5.0 g

Glucose 2.5 g

Beef extract 1.2 g

Tween-80 1.0 ml

K₂HPO₄ 2.0 g

Na-acetate 5.0 g

NH₄-citrate 2.0 g

MgSO₄ × 7 H₂O 0.2 g

MnSO₄ × 4 H₂O 0.05 g

Agar 5.0 g

Distilled water 1000.0 ml

pH 6.0-6.5

Autoclave at 111°C for 30 min.