91. THERMODESULFOBACTERIUM MEDIUM

Solution 1:

Na₂SO₄ 3.0 g

NH₄Cl 1.0 g

MgCl₂ × 6 H₂O 0.2 g

KH₂PO₄ 0.3 g

Na₂HPO₄ × 12 H₂O 2.0 g

FeSO₄ × 7 H₂O 1.5 mg

Resazurin 1.0 mg

Distilled water 930.0 ml

Solution 2:

Trace element solution (see below) 10.0 ml

Solution 3:

Yeast extract 1.0 g

Distilled water 25.0 ml

Solution 4:

Na-lactate 4.0 g

Distilled water 25.0 ml

Solution 5:

Na₂S × 9 H₂O 0.5 g

Distilled water 6.0 ml

Solution 6:

Vitamin solution (see below) 5.0 ml

Trace element solution:

Nitrilotriacetic acid 12.8 g

FeCl₂ × 4 H₂O 0.2 g

MnCl₂ × 4 H₂O 0.1 g

CoCl₂ × 6 H₂O 0.17 g

CaCl₂ × 2 H₂O 0.1 g

ZnCl₂ 0.1 g

CuCl₂ 0.02 g

Na₂MoO₄ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.026 g

NaCl 1.0 g

Na₂SeO₃ × 5 H₂O 0.02 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

Ca DL-pantothenate 5.0 mg

Vitamin B₁₂ 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 6.8-7.0

Solutions 1, 3, 4 and 5 autoclave under N₂ at 121°C for 15 min.

Solution 6 is filter sterilized. Before use, neutralize solution 5 by dropwise of 1 N HCl.

 

Updated 01.02.2024