94. MEDIUM FOR CARBON MONOOXIDE OXIDIZERS

Na₂HPO₄ × 12 H₂O 4.5 g

KH₂PO₄ 0.75 g

NH₄Cl 1.5 g

MgSO₄ × 7 H₂O 0.2 g

CaCl₂ × 2 H₂O 0.03 g

Fe(NH₄)-citrate 0.018 g

Agar (if necessary) 1.2 g

Trace element solution SL-6 (see below) 1.0 ml

Vitamin solution (see below) 10.0 ml

Distilled water 1000.0 ml

Trace element solution SL-6:

ZnSO₄ × 7 H₂O 0.1 g

MnCl₂ × 4 H₂O 0.03 g

H₃BO₃ 0.3 g

CoCl₂ × 6 H₂O 0.2 g

CuCl₂ × 2 H₂O 0.01 g

NiCl₂ × 6 H₂O 0.02 g

Na₂MoO₄ 0.03 g

Distilled water 1000.0 ml

Vitamin solution:

Biotin 2.0 mg

Folic acid 2.0 mg

Pyridoxine-HCl 10.0 mg

Thiamine-HCl × 2 H₂O 5.0 mg

Riboflavin 5.0 mg

Nicotinic acid 5.0 mg

D-Ca-pantothenate 5.0 mg

Vitamin B₁₂ 0.1 mg

p-Aminobenzoic acid 5.0 mg

Lipoic acid 5.0 mg

Distilled water 1000.0 ml

pH 7.0

Autoclave at 121°C for 15 min, add vitamin solution sterilized by filtration. For chemoautotrophic growth incubate under gas atmosphere of a) 20-80% CO₂ + 10% O₂ + 0-70% N₂ or b) 70% H₂ + 20% O₂ + 10% CO₂ adding 2.5 g NaHCO₃ per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air.

 

Updated 01.02.2024